Atrophy of myoepithelial cells in parotid glands of diabetic mice; detection using skeletal muscle actin, a novel marker☆
نویسندگان
چکیده
In mouse parotid glands, we found expression of skeletal muscle actin (actin-α1) protein and mRNA. We isolated myoepithelial cells from the mouse parotid glands and investigated their actin-α1 expression because smooth muscle actin (actin-α2) has been used as a marker for myoepithelial cells. We used actin-α1 expression to identify pathological changes in diabetic non-obese diabetic (NOD; NOD/ShiJcl) mice-a mouse model for Sjögren's syndrome-and found myoepithelial cells to be decreased or atrophied in the diabetic state.
منابع مشابه
The Effect of Resistance Training and Endothelial Stem Cell Injection on Βeta-Actin, Phosphorylated and Total AKT of Skeletal Muscle in Type 1 Diabetic Rats
Background: Type 1 diabetes is associated with decreased skeletal muscle capillary and improper regulation of angiogenesis pathways in skeletal muscle. This research intended to study the effect of resistance training and endothelial stem cell injection on βeta-actin, phosphorylated and total AKT of skeletal muscle in type 1 diabetic rats. Methods: In this experimental study, 36 male Wistar ra...
متن کاملIsolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution
Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...
متن کاملProliferative Activity of Myoepithelial Cells in Irradiated Rabbit Parotid and Submandibular Salivary Glands
BACKGROUND The behavior of salivary myoepithelial cells (MEC) during chronic irradiation exposure is unknown. This study aimed to investigate the response of MEC to prolonged radiation exposure. MATERIALS AND METHODS 16 rabbits and four controls were irradiated with either 10 Gy, 20 Gy, 30 Gy or 40 Gy (Gray units) of direct axial beam radiation. Parotid and submandibular glands were removed a...
متن کاملExpression of Podoplanin and Classical Cadherins in Salivary Gland Epithelial Cells of Klotho-Deficient Mice
We have recently shown that salivary gland myoepithelial cells express podoplanin. Podoplanin indirectly binds the actin filament network which links classical cadherins. The study here is aimed to investigate the expression of podoplanin and cadherins on salivary gland myoepithelial cells and the changes in the aging cells using klotho-deficient (kl/kl) mice. The submandibular glands of kl/kl ...
متن کاملImmunoelectron Microscopic Study of Podoplanin Localization in Mouse Salivary Gland Myoepithelium
We have recently reported that salivary gland cells express the lymphatic endothelial cell marker podoplanin. The present study was aimed to immunohistochemically investigate the expression of the myoepithelial cell marker α-smooth muscle actin (SMA) on podoplanin-positive cells in mouse parotid and sublingual glands, and to elucidate podoplanin localization in salivary gland myoepithelial cell...
متن کامل